The Ambient Calculus has been recently proposed as a model of mobility of agents in a dynamically changing hierarchy of domains. In this paper, we describe the implementation of the theory and metatheory of Ambient Calculus and its modal logic in the Calculus of Inductive Constructions. We take full advantage of Higher-Order Abstract Syntax, using the Theory of Contexts a fundamental tool for developing formally the metatheory of the object system. Among others, we have successfully proved a set of fresh renamings properties, and formalized the connection between the Theory of Contexts and Gabbay-Pitts' “new” quantifier. As a feedback, we introduce a new definition of satisfaction for the Ambients logic and derive some of the properties originally assumed as axioms in the Theory of Contexts. © 2002 Published by Elsevier Science B. V.